Accuracy in cholesterol assays.

Accuracy in cholesterol assays.

Comparison of two assays for measuring LDL cholesterol.

The purpose of this study was to evaluate the LipiDirect assay (L-LDL) against the Direct LDL immunoseparation method (D-LDL) and beta quantification (BQ-LDL) for measurement of LDL cholesterol (LDL-C) in patients with normo- and hypertriglyceridemia. Samples from 156 patients [triglyceride (Tg) range 0.61-9.95 g/L] were assayed for LDL-C concentrations with the three methods. An additional sev...

The Effect of Sample Storage on Total Cholesterol and Hdl-cholesterol Assays

The result of sample analysis is said to be as good as the sample collection and preparation. Without adequate sample handling, accuracy and precision of sample analysis will be greatly affected. The effect of sample storage temperature and length of storage time on serum total cholesterol (TC) and HDL-cholesterol (HDL-C) assays was studied using twenty different samples, pooled into three. Eac...

Accuracy, precision, and quality control of enzyme assays.

25-Hydroxyvitamin D assays: the quest for accuracy.

The recently reported difficulties experienced by Quest Laboratories (1 ) have once again highlighted concerns about the reliability of 25-hydroxyvitamin D (25-OHD) results, particularly those generated by liquid chromatography–tandem mass spectrometry (LC-MS/ MS), the method used by Quest. In the New York Times article of January 8, 2009 (1 ), a spokesman for Quest admitted that some erroneous...